Journal: Mutation research

Article Title: Mutagenic potential of hypoxanthine in live human cells

doi: 10.1016/j.mrfmmm.2017.06.005

Figure Lengend Snippet: A) Structures of deoxyadenosine (dA) and deoxyinosine (dI). B) The SV40-containing replicating shuttle vector used for the construction of plasmid DNA containing a site-specific Hx lesion. C) The target sequence containing Hx lesion at EcoRI site in the leading strand (pBSII(KS+)-SV40) and the lagging strand (pBSII(KS−)-SV40) are shown with respect to SV40 origin.

Article Snippet: DNA methylation was tested by transforming the methylated plasmid DNA with or without DpnI (New England Biolabs, MA) digestion into XL-1 Blue followed by colony formation.

Techniques: Plasmid Preparation, Sequencing

Journal: Mutation research

Article Title: Mutagenic potential of hypoxanthine in live human cells

doi: 10.1016/j.mrfmmm.2017.06.005

Figure Lengend Snippet: A) OGG1 and APE1 digestion of undamaged and 8-oxo-G containing pBSII-sv40ori plasmids. Upon treatment with OGG1 and APE1, 8-oxo-G-containing CCC DNA is converted to nicked DNA. See Materials and methods for details. B) Replication efficiency of undamaged and 8-oxo-G-containing plasmid DNA. C) 8-oxo-G-induced mutation frequency. D) 8-oxo-G-induced mutation distributions. CCC: covalently closed circular DNA; 8-oxo-G: 7,8-Dihydro-8-oxoguanine. *p=0.00342.

Article Snippet: DNA methylation was tested by transforming the methylated plasmid DNA with or without DpnI (New England Biolabs, MA) digestion into XL-1 Blue followed by colony formation.

Techniques: Plasmid Preparation, Mutagenesis

Journal: Mutation research

Article Title: Mutagenic potential of hypoxanthine in live human cells

doi: 10.1016/j.mrfmmm.2017.06.005

Figure Lengend Snippet: A) MPG and APE1 digestion of undamaged and Hx-containing pBSII-sv40ori plasmids. Upon treatment with MPG and APE1, Hx-containing CCC DNA is converted to nicked DNA. See Materials and methods for details. B) Replication efficiency of undamaged and Hx-containing plasmid DNA. C) Hx-induced mutation frequency. D) Hx-induced mutation distributions. CCC: covalently closed circular DNA. *p≤0.02, ****p<0.0001.

Article Snippet: DNA methylation was tested by transforming the methylated plasmid DNA with or without DpnI (New England Biolabs, MA) digestion into XL-1 Blue followed by colony formation.

Techniques: Plasmid Preparation, Mutagenesis

Journal: Mutation research

Article Title: Mutagenic potential of hypoxanthine in live human cells

doi: 10.1016/j.mrfmmm.2017.06.005

Figure Lengend Snippet: A) Replication efficiency of undamaged and Hx-containing plasmid DNA. B) Hx-induced mutation frequency. C) Hx-induced mutation distributions.*p=0.02, ***p≤0.0007.

Article Snippet: DNA methylation was tested by transforming the methylated plasmid DNA with or without DpnI (New England Biolabs, MA) digestion into XL-1 Blue followed by colony formation.

Techniques: Plasmid Preparation, Mutagenesis